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Uniformity of Illumination with TOFRA 030-385 LED Light Source

Tests were performed on Olympus BX51 microscope with 10X UPlanFL 0.3NA and 20X UPlanFL 0.5NA objectives and Chroma 39000 - AT - DAPI/Hoechst/AlexaFluor 350 filter cube.

Camera - Basler color camera scA 1400-17gc, chip size 1390*1038 pixels, pixel size 6.45 μm * 6.45 μm, in 8-bit mode.  Gain 200.

Intensity profiles were drawn through the center of the frame and averaged over 20 rows (columns).

Specimen: Chroma Blue Autofluorescent Plastic Slide.

Field of View	Horizontal Intensity Profile	Vertical Intensity Profile
10X: Exposure time 80 μsec.
20X: Exposure time 45 μsec.