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Imaging of Fluorescent Beads with TOFRA 10W White LED Light Source

Tests were performed on an Olympus BX51 microscope with a 20X UPlanFL 0.5NA objective and 40X UPlanFL 0.75NA objective.

Filter cube for fluorescence - Chroma 39002 - AT - EGFP/FITC/Cy2/AlexaFluor 488.

Camera - Basler color camera SCA 1400-17gc, chip size 1390*1038 pixels, pixel size 6.45 μm * 6.45 μm, in 8-bit mode.

Specimen - Spherotech Fluorescent Particle Slide, Ultra Rainbow, 5 Intensities, 5.05 μm, Cat. No. FPS-5057-UR5.

 

1. Full camera frame with a mixture of beads at 20X. Exposure time 30 ms.

2. Full camera frame with a mixture of beads at 40X.  Exposure time 30 ms.