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Uniformity of Illumination with TOFRA 10W White LED Light Source

Tests were performed on Olympus BX51 microscope with 10X UPlanFL 0.3NA objectives and Chroma 39002 AT filter cube.

Camera - Basler color camera SCA 1400-17gc, chip size 1390*1038 pixels, pixel size 6.45 μm * 6.45 μm, in 8-bit mode.

Specimen - Chroma Green Autofluorescent Plastic Slide.

Intensity profiles were drawn through the center of the frame and averaged over 20 rows (columns).

Field of View

Horizontal Intensity Profile

Vertical Intensity Profile

10X: Exposure time 150 μsec. Green component of the color image is used for plotting.